ScanCol24-train&data: --------------------- This example should be run once you understand how to prepare and analyze image coming from a flatbed scanner (see ScanCol24-example). These data are provided by Xabier Irigoien from AZTI. This is plancton from net tows in the Bay of Biscay. It is stained and digitized with an HP scanner in reflective mode at 600dpi. There is also an example of a very simple training set in _train subdirectory (only five groups are used). You can explore it with XnView (Apps -> Image viewer (XnView)) and try the different classification algorithms on it. Once you have a satisfactory training set, you can save it for further reuse (Objects -> Save), and you can process the 2 samples provided too. To run this example: -------------------- - Download and unzip "ScanCol24-train&data.zip" in your 'ZooPhytoImage Examples' directory, or anywhere you like to place it. - Start Zoo/PhytoImage and import the training set (sixth button). Select the _train subdirectory. At the end of the importation, you see a table of summary statistics about this training set. - Train a classifier with it (seventh button). Experiment with the different algorithms provided, and display the "confusion matrix" using the eighth button after the training. - Train a classifier with this training set and save it (Objects -> save; to reuse it later, or on another computer, reopen the corresponding file with Objects -> load). Inspect its performances. For the sake of this tutorial, we will assume that you are satisfied with it. - Now that you have a classifier, you can predict all particules in your two samples, and calculate total/partial abundances, total/partial biomasses and total/partial size spectra. Usually, you need to document your series in a 'description.zis' file (nineth button) before you can process it. In the present case, we provide an example description file. Inspect it to learn what you are supposed to put in it. - For processing the two samples, click on the tenth button and select the provided 'description.zis' file. Each sample is processed in turn. If you checked the option for saving individual measurements, a table for each sample with the ECD, identification and calculation of individual biomass of each particle is saved on the same directory as the one where the 'description.zis' file resides. Once this is done, you can visualize the results by clicking on the eleventh button. - Finally, you can further analyse your series by using all the statistical or graphical tools available in R. See Help -> Manuals -> An Introduction to R from the 'R Console' menu, if you are not familiar with R. All the data is in a 'ZIRes' object (called 'ZIres' by default), and it is a 'data frame' in R's terminology. Since this is the basic component in R, you can easily use any function (like plot(), for instance) on it to continue your analysis with R. - If you prefer to use another software for you analysis (Matlab? Excel? other?) you just need to export your data (twelveth button). Data are exported as tab separated ASCII files, a format easily readable by any external software.